Composite

Part:BBa_K3102040

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)


T7-RBS-MDH-Ter

This T7-RBS-MDH-ter composite part is made of the MDH enzyme allowing the reversible oxidation of malate oxaloacetate. This reaction is coupled with an NAD+ reduction, generating electrons that will be used to generate an electrical current.

This construct is enriched with T7 promoter, RBS, and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 299
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 143
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None